Institutioner vid LTH - Forskningsoutput - Lunds universitet

Faculty of Engineering, LTH - Research Outputs - Lund

The transglycosylation activity of CGTase is well reported, as it is able to glucosylate other phenolic compounds such as resveratrol [ 26, 27 ], catechin [ 28 ], hydroquinone [ 29 ], kaempferol [ 30] or genistein [ 31 ]. Table 1. Cyclodextrin glucanotransferase (CGTase) from Bacillus macerans was applied to produce the CDs from linear α- (1,4)-glucans, which were obtained by Neisseria polysaccharea amylosucrase (Np AS) treatment on sucrose. The greatest CD yield (21.1%, w/w) was achieved from a one-pot dual enzyme reaction at 40 °C for 24 h. Amyloglucosidase from Aspergillus niger (Spirizyme Fuel) and CGTase from Thermoanaerobacter sp. (Toruzyme 3.0 L) were kindly donated by Novozymes A/S. CGTase from Bacillus macerans (CGTase Amano) was kindly supplied by Amano Enzyme Inc. Pure amyloglucosidase from Aspergillus niger (ref. 10115) was purchased from Sigma.

Cgtase amano

Glucoamylase was from Toyobo Co., Ltd. (Osaka, Japan) and B. macerans CGTase was from Amano Enzyme Inc. (Aichi, Japan). The enzyme was further purified using DEAE Sepharose Fast Flow media (Amersham Biosciences Europe GmbH). The enzyme was eluted with a linear gradient between 0 and 0.5 M NaCl in Tris–HCl buffer, pH 7.8. CGTase (from Paenibacillus macerans) was purchased from Amano Enzyme Inc. (Aichi, Japan).

Faculty of Engineering, LTH - Research Outputs - Lund

(CGTase). Feruloil Esterasa. cyclomaltodextrin glycosyltransferase; konchizaimu; α-1,4-glucan 4- glycosyltransferase, cyclizing; BMA; CGTase; neutral-cyclodextrin glycosyltransferase; 31 Jul 1998 Ciclodextrina glucosiltransferase (CGTase E. C. 2.4.1.19) de Bacillus macerans da Amano International Enzyme Co. Inc. (Nagoya, Japão), com recubiertos con PEI de distinto tamaño…………………………. 98 modification of cyclodextrin glycosyltransferase (CGTase) from.

Institutioner vid LTH - Forskningsoutput - Lunds universitet

ATCC 53627, Bacillus stearothermophilus and a Carboxydocella sp. (phylogenetically identified from genomic DNA) were characterized with respect to their catalytic activity in different reactions, with emphasis on reactions useful for the elongation of the carbohydrate group of alkyl glycosides. glycosyltransferase (CGTase) gave rise to a signiﬁcant formation of glycosylated products. The enzyme from Thermoanaerobacter sp.

Food Chemistry, 138(2-3), glycosyltransferase (CGTase) catalyzed synthesis of alkyl A CGTase with high coupling activity commercial CGTase from Amano Enzyme Inc [120]. ever, such reports on the production of CGTase by immo bilized cells are [22} J.C. Ogbonna, Y. Amano, H. Nakamura, Elucidation of optimum conditions for (cyclomaltodextrin glucanotransferasa, CGTase). Existen tres CD con Estas moléculas, deberán tener un tamaño compatible con la cavidad interna de la CD, . of cyclodextrin glucosyltransferase (CGTase, EC 2.4.1.19) on hydrolyzed starch.
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(phylogenetically identified from genomic DNA) were characterized with respect to their catalytic activity in different reactions, with emphasis on reactions useful for the elongation of the carbohydrate group of alkyl glycosides. glycosyltransferase (CGTase) gave rise to a signiﬁcant formation of glycosylated products. The enzyme from Thermoanaerobacter sp. (Toruzyme 3.0L) worked better than that from Bacillus macerans (CGTase Amano).

Cyclomaltodextrin glucanotransferase (CGTase) (from Paenibacillus macerans) was provided by Amano Enzyme Inc. (Nagoya, Japan). All other chemicals used were from commercial sources and were ana-lytical grade. Microorganisms and medium. V. dahliae TPU 4900, Bacillus cereus TPU 5504, 2017-07-18 The present invention provides a method for producing isoquercitrin, α-glycosylisoquercitrin, and rhamnose, the method comprising a step of naringin-degrading enzyme treatment during the isoquercitrin production from rutin in the presence of an edible component, such as gelatin, wheat gluten, chitosan, lecithin, a glycerol fatty acid ester, xanthan gum, carrageenan, sodium chondroitin sulfate 例えば、ブレビバクテリウム（Brevibacterium）属の生産するγ-CGTaseが用いられる。【選択図】なし JP2005312464A - 配糖体及び糖転移生成物の製造法 - Google Patents 配糖体及び糖 2005-07-25 Application filed by Amano Enzyme Inc, As Amano CGTase also has a high coupling activity, other positions must also influence the ability to catalyze the coupling reaction, and, e.g., the variations in subsites +1 and −7 could be of interest.
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Faculty of Engineering, LTH - Research Outputs - Lund

Cyclodextrin glucanotransferase from B. macerans (CGTase, EC 2.4.1.19) with activity of 600 U/ml was purchased from Amano Enzyme Inc., Japan. Water soluble potato starch was purchased from Sigma, Malaysia. Isolation of CNF support The isolation process of CNF consists of several steps of alkaline process and high-intensity ultrasonication. Kenaf A produção de CDs foi feita usando a enzima ciclodextrina glucosiltransferase (CGTase E. C. 2.4.1.19), proveniente de Bacillus macerans, da Amano Intemational Enzyme, a 50°C e a avaliação dos resultados foi feito através de HPLC (High Performance Chromatography Liquid). amylase and a CGTase are combined more glucose is generated from starch than would obtained from AMANO International Enzyme Co., Inc. (USA).

Institutioner vid LTH - Forskningsoutput - Lunds universitet

ATCC 53627 CGTase (Toruzyme 3.0L,) from Novozymes (Bagsvaerd, Denmark). These enzymes have been In enzymology, a cyclomaltodextrin glucanotransferase (also cyclodextrin glycosyl transferase or CGTase for short) (EC 2.4.1.19) is an enzyme that catalyzes the chemical reaction of cyclizing part of a 1,4-alpha-D-glucan molecule through the formation of a 1,4-alpha-D-glucosidic bond. BM CGTase was obtained from Amano Enzyme Inc. (Aichi, Japan) and had a specific activity of 1003 U·mg −1 of dextrinizing activity []. A11 CGTase was purified using starch adsorption and ion exchange chromatography (DEAE‐Toyopearl 650M column; Tosoh Corporation, Tokyo, Japan). CGTase could be successfully immobilised into a nanofibrous polyvinyl alcohol (PVA) membrane (Saallah et al., 2016) by co-electrospinning of CGTase and PVA mixture, which allow the enzyme to attach andencapsulatedinthenanofibers.ThePVAsolution is rich with hydroxyl groups that enable the formation of secondary bonding with the enzyme molecules. 1996-01-01 · CGTase, which produces mainly ~-CD, seems to exist widely. The number of ~'-CD producing enzyme is increasing, partly because of ~'-CD's high solubility, its large cavity and its potential usefulness.

-CD was purchased from Sigma-Aldrich (M) Sdn. Bhd. (USA).